Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production | |
Zhang, Yu1,2,3 ; Zhang, Xiaohuan1,2,3,4; Yu, Qiang1,2,3; Yuan, Zhenhong1,2,3; Chen, Xiaoyan1,2,3,5
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| 2019-12-01 | |
| Source Publication | JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
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| ISSN | 1556-6560 |
| Volume | 13Issue:6Pages:799-805 |
| Corresponding Author | Yu, Qiang(yuqiang@ms.giec.ac.cn) |
| Abstract | Itaconic acid (IA) is one of the most important biobased chemicals, and is generally produced by the filamentous fungi Aspergillus terreus. In order to degrade the production cost of IA, the low-cost and renewable lignocelluloses could be used for IA production. However, inhibitors including furfural derived from indispensable lignocelluloses pretreatment, can seriously hinder the microbial growth and fermentation, and microbial IA production is also no exception. Compared to Saccharomyces cerevisiae, less is known about the effects of furfural for Aspergillus terreus. Here, a furfural tolerant strain was obtained by chemical mutagenesis with diethyl sulfate (DES), and the highest furfural tolerable concentration was up to 1.3 g/L in solid medium. The growth, glucose consumption and IA production of mutant and parental strain were compared in fermentation media containing different furfural concentration, which found 0.5 g/L furfural is the critical concentration. In addition, the morphology of organelle structure was observed with transmission electron microscope and the cellular sugar content was quantified to further support the changes in cell morphology and the impact of furfural. |
| Keyword | Aspergillus terreus Furfural Tolerance Mutagenesis TEM |
| DOI | 10.1166/jbmb.2019.1909 |
| WOS Keyword | SACCHAROMYCES-CEREVISIAE ; ACETIC-ACID ; ENHANCED TOLERANCE ; FERMENTATION ; XYLOSE ; CELLS |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[21506215] ; National Natural Science Foundation of China[51876206] ; Municipal Science and Technology Project of Guangzhou (China)[201804010081] |
| WOS Research Area | Chemistry ; Energy & Fuels ; Materials Science |
| Funding Organization | National Natural Science Foundation of China ; Municipal Science and Technology Project of Guangzhou (China) |
| WOS Subject | Chemistry, Applied ; Energy & Fuels ; Materials Science, Biomaterials |
| WOS ID | WOS:000482706800008 |
| Publisher | AMER SCIENTIFIC PUBLISHERS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/25478 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Yu, Qiang |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.SUNY Coll Environm Sci & Forestry, Dept Paper & Bioproc Engn, Syracuse, NY 13210 USA |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zhang, Yu,Zhang, Xiaohuan,Yu, Qiang,et al. Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production[J]. JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,2019,13(6):799-805. |
| APA | Zhang, Yu,Zhang, Xiaohuan,Yu, Qiang,Yuan, Zhenhong,&Chen, Xiaoyan.(2019).Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production.JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,13(6),799-805. |
| MLA | Zhang, Yu,et al."Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production".JOURNAL OF BIOBASED MATERIALS AND BIOENERGY 13.6(2019):799-805. |
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