Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer | |
| Yu Jia-Jun1; Liu Ping3; Zeng Zhen1; Chen Ying1; Gao Wei1,2; Li Mei1,2; Wang Chen-Guang4; Huang Zheng-Xu1,2; Zhou Zhen1,2; Li Lei1,2 | |
| 2018-04-01 | |
| Source Publication | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| ISSN | 0253-3820 |
| Volume | 46Issue:4Pages:463-470 |
| Corresponding Author | Li Lei(lileishdx@163.com) |
| Abstract | Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an important analytical technique for biological macromolecules, such as proteins, peptides and nucleic acid, especially in the field of microbial identification. On the basis of previous study, a linear MALDI-TOF MS was been designed and assembled for biological applications. The instrument comprised a vacuum system, a vacuum fast sample introduction system, an optical system, a time-of-flight mass analyzer, an ion source, a data acquisition system and an electronic control system. The ion source adopted two-stage source acceleration, delayed extraction and dynamic pulse focusing technique. The time-of-flight distance of field-free drift region was about 1 m. The optical system adopted a solid-state laser with adjustable frequency of 1-2000 Hz and spots of 20-100 mu m. The angle of incidence laser was controlled at 5 degrees. A series of experiments were carried out to further evaluate the instrument performances. It could not only analyze the samples more than 199 kDa, but also achieve isotope resolution at 1000-3000 Da and up to 900 (FWHM) at 5000-17000 Da. The minimum detectable concentration of gramicidin was 10 amol mu L-1, absolute sensitivity reached up to 2.56 amol. Independent detection of saliva samples from different targets showed that the instrument had higher producibility. We identified Escherichia coli and Shigella spp., which are two common bacteria but difficult to be differentiated by mass spectrometry, showing its potential identification for clinical microorganism. In summary, this instrument can play a role on clinical examination in the near future. |
| Keyword | Matrix-assisted laser desorption ionization Linear time-of-flight mass spectrometer Development Performance characterization |
| DOI | 10.1016/S1872-2040(17)61077-6 |
| WOS Keyword | MALDI-TOF MS ; ESCHERICHIA-COLI ; ION FORMATION ; VITEK MS ; TIME ; IDENTIFICATION ; DESORPTION/IONIZATION ; BIOTYPER ; EXTRACTION ; RESOLUTION |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Guangdong Applied Science and Technology Research and Development, China[2015B020236003] ; Fundamental Research Funds for the Central Universities of China[21616341] |
| WOS Research Area | Chemistry |
| Funding Organization | Guangdong Applied Science and Technology Research and Development, China ; Fundamental Research Funds for the Central Universities of China |
| WOS Subject | Chemistry, Analytical |
| WOS ID | WOS:000434467300002 |
| Publisher | ELSEVIER SCIENCE INC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/23418 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Li Lei |
| Affiliation | 1.Jinan Univ, Inst Mass Spectrometer & Atmospher Environm, Guangzhou 510632, Guangdong, Peoples R China 2.Guangdong Prov Engn Res Ctr On Line Source Apport, Guangzhou 510632, Guangdong, Peoples R China 3.Guangzhou HeXin Instrument Co LTD, Guangzhou 510530, Guangdong, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China |
| Recommended Citation GB/T 7714 | Yu Jia-Jun,Liu Ping,Zeng Zhen,et al. Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2018,46(4):463-470. |
| APA | Yu Jia-Jun.,Liu Ping.,Zeng Zhen.,Chen Ying.,Gao Wei.,...&Li Lei.(2018).Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,46(4),463-470. |
| MLA | Yu Jia-Jun,et al."Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 46.4(2018):463-470. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment