Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva | |
Shang, Changhua1 ; Zhu, Shunni1; Qin, Lei1; Alam, Mohammad A.1; Wang, Zhongming1; Yuan, Zhenhong1,2; Xie, Jun3
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| 2017-10-01 | |
| Source Publication | JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
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| ISSN | 1556-6560 |
| Volume | 11Issue:5Pages:483-490 |
| Corresponding Author | Yuan, Zhenhong(yuanzh@ms.giec.ac.cn) |
| Abstract | Dunaliella parva grew in different nitrogen concentrations, which were associated with lipid accumulation. D. parva could accumulate lipids. However, the underlying mechanism of lipid degradation requires further research. Thus, it is important to analyze lipid degradation to elucidate lipid accumulation. In this report, a gene encoding lipase from the green alga D. parva was isolated and characterized. Experimental results showed that the full-length cDNA of lipase gene was 4,583 bp, with an open reading frame of 3,183 bp, a 73 bp 5'-untranslated region and a 1,327 bp 3'-untranslated region. Potential regulatory elements related to hormones, light and unfavorable environments were found in the 1,828 bp 5'-flanking region. Analysis of lipase gene expression showed a correlation between lipase gene expression and nitrogen concentrations, which indicated that lipase gene may be responsive to nitrogen limitation stress. The cloning and expression analysis of lipase gene provided a basis for studying the regulatory mechanism of lipid degradation and the massive accumulation of lipids or lipase in D. parva. |
| Keyword | Differential Expression Dunaliella parva Lipase Lipid Degradation Nitrogen Limitation |
| DOI | 10.1166/jbmb.2017.1691 |
| WOS Keyword | LED LIGHT WAVELENGTHS ; DOMAIN TRIACYLGLYCEROL LIPASE ; INTENSITY SUPPLY STRATEGIES ; MICROALGAE CHLORELLA-SP ; CHLAMYDOMONAS-REINHARDTII ; BIODIESEL PRODUCTION ; NUTRIENT REDUCTION ; BIOGAS UPGRADE ; PURIFICATION ; PERFORMANCE |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Natural Science Foundation of Guangdong Province[2016A030313171] ; Natural Science Foundation of Guangdong Province[2016A030312007] ; National Natural Sciences Foundation of China[51476177] ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences[y507j21001] |
| WOS Research Area | Chemistry ; Energy & Fuels ; Materials Science |
| Funding Organization | Natural Science Foundation of Guangdong Province ; National Natural Sciences Foundation of China ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences |
| WOS Subject | Chemistry, Applied ; Energy & Fuels ; Materials Science, Biomaterials |
| WOS ID | WOS:000416103100013 |
| Publisher | AMER SCIENTIFIC PUBLISHERS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/16776 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Yuan, Zhenhong |
| Affiliation | 1.Chinese Acad Sci, Key Lab Renewable Energy, Guangdong Key Lab New & Renewable Energy Res & De, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.Collaborat Innovat Ctr Biomass Energy, Zhengzhou 450002, Henan, Peoples R China 3.South China Agr Univ, Inst New Energy & New Mat, Key Lab Energy Plants Resource & Utilizat, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China |
| Recommended Citation GB/T 7714 | Shang, Changhua,Zhu, Shunni,Qin, Lei,et al. Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva[J]. JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,2017,11(5):483-490. |
| APA | Shang, Changhua.,Zhu, Shunni.,Qin, Lei.,Alam, Mohammad A..,Wang, Zhongming.,...&Xie, Jun.(2017).Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva.JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,11(5),483-490. |
| MLA | Shang, Changhua,et al."Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva".JOURNAL OF BIOBASED MATERIALS AND BIOENERGY 11.5(2017):483-490. |
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