3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum

doi:10.1007/s12033-016-9929-y

GIEC OpenIR > 中国科学院广州能源研究所

	3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum
	Xiao, Shiyuan 1,2; Xu, Jingliang1 ; Chen, Xiaoyan 1; Li, Xiekun 1,2; Zhang, Yu1 ; Yuan, Zhenhong1
	2016-05-01
发表期刊	MOLECULAR BIOTECHNOLOGY
卷号	58 期号:5 页码:311-318
摘要	Biofuel offers a promising solution to the adverse environmental problems and depletion in reserves of fossil fuels. Higher alcohols including 3-methyl-1-butanol were paid much more attention as fuel substitute in recent years, due to its similar properties to gasoline. In the present work, 3-methyl-1-butanol production in engineered Corynebacterium glutamicum was studied. alpha-Ketoisovalerate decarboxylase gene (kivd) from Lactococcus lactis combined with alcohol dehydrogenase gene (adh2, adhA, and adh3) from three organisms were overexpressed in C. glutamicum. Enzymatic assay and alcohol production results showed that adh3 from Zymomonas mobilis was the optimum candidate for 3-methyl-1-butanol production in C. glutamicum. The recombinant with kivd and adh3 could produce 0.182 g/L of 3-methyl-1-butanol and 0.144 g/L of isobutanol after 12 h of incubation. Further inactivation of the E1 subunit of pyruvate dehydrogenase complex gene (aceE) and lactic dehydrogenase gene (ldh) in the above C. glutamicum strain would improve the 3-Methyl-1-butanol titer to 0.497 g/L after 12 h of incubation.
文章类型	Article
关键词	3-methyl-1-butanol Corynebacterium Glutamicum Alpha-ketoisovalerate Decarboxylase Alcohol Dehydrogenase Gene Knockout
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s12033-016-9929-y
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	PYRUVATE-DEHYDROGENASE COMPLEX ; ESCHERICHIA-COLI ; AMINO-ACIDS ; DECARBOXYLASES ; FERMENTATION ; MUTAGENESIS ; ALCOHOLS ; ENZYME ; GENE
收录类别	SCI
语种	英语
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000374286500001
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/11237
专题	中国科学院广州能源研究所
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,et al. 3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum[J]. MOLECULAR BIOTECHNOLOGY,2016,58(5):311-318.
APA	Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,Li, Xiekun,Zhang, Yu,&Yuan, Zhenhong.(2016).3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum.MOLECULAR BIOTECHNOLOGY,58(5),311-318.
MLA	Xiao, Shiyuan,et al."3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum".MOLECULAR BIOTECHNOLOGY 58.5(2016):311-318.