Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| 3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum | |
Xiao, Shiyuan1,2; Xu, Jingliang1 ; Chen, Xiaoyan1; Li, Xiekun1,2; Zhang, Yu1; Yuan, Zhenhong1
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| 2016-05-01 | |
| Source Publication | MOLECULAR BIOTECHNOLOGY
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| Volume | 58Issue:5Pages:311-318 |
| Abstract | Biofuel offers a promising solution to the adverse environmental problems and depletion in reserves of fossil fuels. Higher alcohols including 3-methyl-1-butanol were paid much more attention as fuel substitute in recent years, due to its similar properties to gasoline. In the present work, 3-methyl-1-butanol production in engineered Corynebacterium glutamicum was studied. alpha-Ketoisovalerate decarboxylase gene (kivd) from Lactococcus lactis combined with alcohol dehydrogenase gene (adh2, adhA, and adh3) from three organisms were overexpressed in C. glutamicum. Enzymatic assay and alcohol production results showed that adh3 from Zymomonas mobilis was the optimum candidate for 3-methyl-1-butanol production in C. glutamicum. The recombinant with kivd and adh3 could produce 0.182 g/L of 3-methyl-1-butanol and 0.144 g/L of isobutanol after 12 h of incubation. Further inactivation of the E1 subunit of pyruvate dehydrogenase complex gene (aceE) and lactic dehydrogenase gene (ldh) in the above C. glutamicum strain would improve the 3-Methyl-1-butanol titer to 0.497 g/L after 12 h of incubation. |
| Subtype | Article |
| Keyword | 3-methyl-1-butanol Corynebacterium Glutamicum Alpha-ketoisovalerate Decarboxylase Alcohol Dehydrogenase Gene Knockout |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s12033-016-9929-y |
| WOS Subject Extended | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS Keyword | PYRUVATE-DEHYDROGENASE COMPLEX ; ESCHERICHIA-COLI ; AMINO-ACIDS ; DECARBOXYLASES ; FERMENTATION ; MUTAGENESIS ; ALCOHOLS ; ENZYME ; GENE |
| Indexed By | SCI |
| Language | 英语 |
| WOS Subject | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS ID | WOS:000374286500001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/11237 |
| Collection | 中国科学院广州能源研究所 |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,et al. 3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum[J]. MOLECULAR BIOTECHNOLOGY,2016,58(5):311-318. |
| APA | Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,Li, Xiekun,Zhang, Yu,&Yuan, Zhenhong.(2016).3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum.MOLECULAR BIOTECHNOLOGY,58(5),311-318. |
| MLA | Xiao, Shiyuan,et al."3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum".MOLECULAR BIOTECHNOLOGY 58.5(2016):311-318. |
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