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Improved methanol tolerance of Rhizomucor miehei lipase based on N?glycosylation within the α-helix region and its application in biodiesel production 期刊论文
Biotechnology for Biofuels, 2021, 卷号: 14, 期号: 1
Authors:  Tian,Miao;  Yang,Lingmei;  Wang,Zhiyuan;  Lv,Pengmei;  Fu,Junying;  Miao,Changlin;  Li,Ming;  Liu,Tao;  Luo,Wen
Favorite  |  View/Download：194/0  |  Submit date：2022/09/20
Rhizomucor miehei lipase  α-Helix region  N?Glycosylation  Methanol tolerance  Biodiesel  
Improved methanol tolerance of Rhizomucor miehei lipase based on N-glycosylation within the alpha-helix region and its application in biodiesel production 期刊论文
BIOTECHNOLOGY FOR BIOFUELS, 2021, 卷号: 14, 期号: 1, 页码: 10
Authors:  Tian, Miao;  Yang, Lingmei;  Wang, Zhiyuan;  Lv, Pengmei;  Fu, Junying;  Miao, Changlin;  Li, Ming;  Liu, Tao;  Luo, Wen
Favorite  |  View/Download：496/0  |  Submit date：2022/09/22
Rhizomucor miehei lipase  alpha-Helix region  N-Glycosylation  Methanol tolerance  Biodiesel  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
Authors:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
Favorite  |  View/Download：383/0  |  Submit date：2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2017, 卷号: 102, 页码: 35-40
Authors:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong;  Huang, Shaowei;  Xv, Jingliang
Favorite  |  View/Download：296/0  |  Submit date：2017/10/13
Directed Evolution  Membrane-blot  Methanol Tolerance  Thermostability  Rhizomucor Miehei Lipases  
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region 期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
Authors:  Wang, Zhiyuan;  Lv, Pengmei;  Luo, Wen;  Yuan, Zhenhong;  He, Dong
Favorite  |  View/Download：186/0  |  Submit date：2016/12/05
Enzyme Property  Intramolecular Chaperone  Propeptide Replacing  Protein Folding  Rhizomucor Miehei Lipases  
米黑根毛霉脂肪酶在毕赤酵母中的高效表达及酶学性质研究 期刊论文
林产化学与工业, 2016, 期号: 1, 页码: 135-140
Authors:  罗文;  王治元;  苗长林;  吕鹏梅;  何东;  李惠文;  杨玲梅;  袁振宏
View  |  Adobe PDF(688Kb)  |  Favorite  |  View/Download：600/151  |  Submit date：2017/12/31
米黑根毛霉脂肪酶  毕赤酵母  密码子优化  酶学性质  
Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase 期刊论文
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 卷号: 42, 期号: 8, 页码: 1175-1182
Authors:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong
Favorite  |  View/Download：158/0  |  Submit date：2016/10/27
Rml  Pichia Pastoris  P-gap  P-aox1  Optimization  
米黑根毛霉脂肪酶基因的克隆、表达及活性分析 期刊论文
生物技术通报, 2013, 期号: 6, 页码: 122
Authors:  苗长林;  罗文;  刘姝娜;  吕鹏梅;  李惠文;  杨玲梅;  袁振宏;  蒋剑春
Favorite  |  View/Download：169/0  |  Submit date：2021/11/01
米黑根毛霉  脂肪酶  毕赤酵母  基因克隆  基因表达  
米黑根毛霉脂肪酶基因的克隆表达及活性分析 期刊论文
生物技术通报, 2013, 期号: 6, 页码: 122
Authors:  苗长林;  罗文;  刘姝娜;  吕鹏梅;  李惠文;  杨玲梅;  袁振宏;  蒋剑春
Favorite  |  View/Download：252/0  |  Submit date：2019/11/30