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Creator:Wang, Zhiyuan
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Document Type:期刊论文
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Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design
期刊论文
BIORESOURCE TECHNOLOGY, 2022, 卷号: 348, 页码: 9
Authors:
Tian, Miao
;
Yang, Lingmei
;
Lv, Pengmei
;
Wang, Zhiyuan
;
Fu, Junying
;
Miao, Changlin
;
Li, Zhibing
;
Li, Lianhua
;
Liu, Tao
;
Du, Wenyi
;
Luo, Wen
Favorite
  |  
View/Download:182/0
  |  
Submit date:2023/11/21
Biodiesel
Methanol tolerance
N-glycosylation
Molecular dynamics
Rhizomucor miehei lipase
Improved methanol tolerance of Rhizomucor miehei lipase based on N-glycosylation within the alpha-helix region and its application in biodiesel production
期刊论文
BIOTECHNOLOGY FOR BIOFUELS, 2021, 卷号: 14, 期号: 1, 页码: 10
Authors:
Tian, Miao
;
Yang, Lingmei
;
Wang, Zhiyuan
;
Lv, Pengmei
;
Fu, Junying
;
Miao, Changlin
;
Li, Ming
;
Liu, Tao
;
Luo, Wen
Favorite
  |  
View/Download:496/0
  |  
Submit date:2022/09/22
Rhizomucor miehei lipase
alpha-Helix region
N-Glycosylation
Methanol tolerance
Biodiesel
Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production
期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
Authors:
Zhang, Jun
;
Chen, Xiaoyan
;
Lv, Pengmei
;
Luo, Wen
;
Wang, Zhiyuan
;
Xu, Jingliang
;
Wang, Zhongming
Favorite
  |  
View/Download:338/0
  |  
Submit date:2021/10/27
Lipase
Bionic-immobilization
Biodiesel
Bio-silicification
Molecular dynamics simulation
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide
期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
Authors:
Tian, Miao
;
Huang, Shaowei
;
Wang, Zhiyuan
;
Fu, Junying
;
Lv, Pengmei
;
Miao, Changlin
;
Liu, Tao
;
Yang, Lingmei
;
Luo, Wen
Favorite
  |  
View/Download:383/0
  |  
Submit date:2021/11/01
Propeptide
RML
Enzyme activity
Directed saturation mutation
High-efficiency expression of the thermophilic lipase from Geobacillus thermocatenulatus in Escherichia coli and its application in the enzymatic hydrolysis of rapeseed oil
期刊论文
3 BIOTECH, 2020, 卷号: 10, 期号: 12, 页码: 9
Authors:
Zhang, Jun
;
Tian, Miao
;
Lv, Pengmei
;
Luo, Wen
;
Wang, Zhiyuan
;
Xu, Jingliang
;
Wang, Zhongming
Favorite
  |  
View/Download:344/0
  |  
Submit date:2021/11/01
Thermophilic lipase
Escherichia coli
Enzymatic hydrolysis
Rapeseed oil
Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability
期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2017, 卷号: 102, 页码: 35-40
Authors:
He, Dong
;
Luo, Wen
;
Wang, Zhiyuan
;
Lv, Pengmei
;
Yuan, Zhenhong
;
Huang, Shaowei
;
Xv, Jingliang
Favorite
  |  
View/Download:296/0
  |  
Submit date:2017/10/13
Directed Evolution
Membrane-blot
Methanol Tolerance
Thermostability
Rhizomucor Miehei Lipases
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region
期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
Authors:
Wang, Zhiyuan
;
Lv, Pengmei
;
Luo, Wen
;
Yuan, Zhenhong
;
He, Dong
Favorite
  |  
View/Download:186/0
  |  
Submit date:2016/12/05
Enzyme Property
Intramolecular Chaperone
Propeptide Replacing
Protein Folding
Rhizomucor Miehei Lipases
Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase
期刊论文
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 卷号: 42, 期号: 8, 页码: 1175-1182
Authors:
He, Dong
;
Luo, Wen
;
Wang, Zhiyuan
;
Lv, Pengmei
;
Yuan, Zhenhong
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  |  
View/Download:158/0
  |  
Submit date:2016/10/27
Rml
Pichia Pastoris
P-gap
P-aox1
Optimization