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Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design 期刊论文
BIORESOURCE TECHNOLOGY, 2022, 卷号: 348, 页码: 9
Authors:  Tian, Miao;  Yang, Lingmei;  Lv, Pengmei;  Wang, Zhiyuan;  Fu, Junying;  Miao, Changlin;  Li, Zhibing;  Li, Lianhua;  Liu, Tao;  Du, Wenyi;  Luo, Wen
Favorite  |  View/Download：182/0  |  Submit date：2023/11/21
Biodiesel  Methanol tolerance  N-glycosylation  Molecular dynamics  Rhizomucor miehei lipase  
Improved methanol tolerance of Rhizomucor miehei lipase based on N-glycosylation within the alpha-helix region and its application in biodiesel production 期刊论文
BIOTECHNOLOGY FOR BIOFUELS, 2021, 卷号: 14, 期号: 1, 页码: 10
Authors:  Tian, Miao;  Yang, Lingmei;  Wang, Zhiyuan;  Lv, Pengmei;  Fu, Junying;  Miao, Changlin;  Li, Ming;  Liu, Tao;  Luo, Wen
Favorite  |  View/Download：496/0  |  Submit date：2022/09/22
Rhizomucor miehei lipase  alpha-Helix region  N-Glycosylation  Methanol tolerance  Biodiesel  
Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production 期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
Authors:  Zhang, Jun;  Chen, Xiaoyan;  Lv, Pengmei;  Luo, Wen;  Wang, Zhiyuan;  Xu, Jingliang;  Wang, Zhongming
Favorite  |  View/Download：336/0  |  Submit date：2021/10/27
Lipase  Bionic-immobilization  Biodiesel  Bio-silicification  Molecular dynamics simulation  
Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2021, 卷号: 150, 页码: 8
Authors:  Tian, Miao;  Huang, Shaowei;  Wang, Zhiyuan;  Fu, Junying;  Lv, Pengmei;  Miao, Changlin;  Liu, Tao;  Yang, Lingmei;  Luo, Wen
Favorite  |  View/Download：383/0  |  Submit date：2021/11/01
Propeptide  RML  Enzyme activity  Directed saturation mutation  
High-efficiency expression of the thermophilic lipase from Geobacillus thermocatenulatus in Escherichia coli and its application in the enzymatic hydrolysis of rapeseed oil 期刊论文
3 BIOTECH, 2020, 卷号: 10, 期号: 12, 页码: 9
Authors:  Zhang, Jun;  Tian, Miao;  Lv, Pengmei;  Luo, Wen;  Wang, Zhiyuan;  Xu, Jingliang;  Wang, Zhongming
Favorite  |  View/Download：344/0  |  Submit date：2021/11/01
Thermophilic lipase  Escherichia coli  Enzymatic hydrolysis  Rapeseed oil  
Establishment and application of a modified membrane-blot assay for Rhizomucor miehei lipases aimed at improving their methanol tolerance and thermostability 期刊论文
ENZYME AND MICROBIAL TECHNOLOGY, 2017, 卷号: 102, 页码: 35-40
Authors:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong;  Huang, Shaowei;  Xv, Jingliang
Favorite  |  View/Download：296/0  |  Submit date：2017/10/13
Directed Evolution  Membrane-blot  Methanol Tolerance  Thermostability  Rhizomucor Miehei Lipases  
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region 期刊论文
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2016, 卷号: 62, 期号: 1, 页码: 25-30
Authors:  Wang, Zhiyuan;  Lv, Pengmei;  Luo, Wen;  Yuan, Zhenhong;  He, Dong
Favorite  |  View/Download：186/0  |  Submit date：2016/12/05
Enzyme Property  Intramolecular Chaperone  Propeptide Replacing  Protein Folding  Rhizomucor Miehei Lipases  
Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase 期刊论文
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2015, 卷号: 42, 期号: 8, 页码: 1175-1182
Authors:  He, Dong;  Luo, Wen;  Wang, Zhiyuan;  Lv, Pengmei;  Yuan, Zhenhong
Favorite  |  View/Download：158/0  |  Submit date：2016/10/27
Rml  Pichia Pastoris  P-gap  P-aox1  Optimization