Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| 绿假单胞菌（Chloropseudomonas sp. G102）及其对硫化物转化的研究 | |
| 廖俐萍 | |
| Thesis Advisor | 王志通 |
| 1994 | |
| Degree Grantor | 中国科学院广州能源研究所 |
| Place of Conferral | 中国科学院广州能源研究所 |
| Degree Name | 硕士 |
| Degree Discipline | 微生物学 |
| Keyword | 绿硫细菌 绿假单胞菌 硫化物的生物氧化 |
| Abstract | 绿硫细菌（Green sulfur bacteria)是一类能从硫化氢等硫化物的氧化中获得电子，在厌氧条件下借助光能同化CO_2的光合细菌（Photosynthetic bacteria），在生物圈的硫循环和环境自净作用中起着重要的作用。本研究从养猪场废水厌氧发酵处理系统的酸化池废水中，富集分离获得1株绿色细菌，在厌氧、光照、含硫化物培养基中生长，细胞呈杆状，革兰氏染色阴性，有单根生鞭毛。经初步鉴定，属绿菌科（Chlorobiaceae）的绿假单胞菌属（Chloropseudomonas），菌株编 号为G102（Chloropseudomonas sp. G102）。在其生理生化特性研究中测定该菌对硫化物的转化能力，结果在46小时内其转化率可达93.44%。在实验室进行的模拟试验表明，该菌的固定化细胞在同样条件下，45小时内的转化率为87.15%；而用容量为1800ml的有机玻璃柱进行的液体循环流动培养试验中，其70小时的硫化物转化率93.92%。实验表明，其他条件（温度，pH，底物浓度）相同的情况下，光照强度及细胞与硫化物接触程度是决定硫化物去除率的两个关键因素，这为绿硫细菌在处理含硫化物废水方面的实际应用提供了实验依据。 |
| Other Abstract | Green sulfur bacteria are the species of the photosynthetic bacteria that carry out an anaer photosynthesis in which oxidated sulfide serves as electron donors and carbon dioxide is photoassimilated, and they play an important role in the biosphere sulfur cycle and the environmental self-purification. We accumulate, isolate and characterize a green bacterium from the acid settler wastewater of the piggery effluent treatment by anaerobic fermentation. It is a Gram- negative rod which has single polar flagellum, and can grow in the illuminated anaerobic sulfide- containing culture. It is, therefore, preliminarily identified to belong to the family Chlorobiaceae, the genus Chloropseudomonas. The strasin is named Chloropseudomonas sp. G102. On the studies of its physiological biochemical characteristic, we measure the strain's capacity of sulfide transformation and its transformation rate in 46 hour is 99.44%. In the same condition, the simulation test in laboratory indicated that the immobilized-cell of strain G102 can transformate 87.15% sulfide in 46 hours. The culture result of liquid circulating flow in polyvinyl chloride (PVC) column showed that the sulfide transformation rate is 93.92% in 70 hours. The experiments indicated that with the same other condition (temperature, pH, and substrate concentration), the light intensity and the contact level of cells and sulfide are two key factors contorlling the sulfide transformation rate. This provide experimental basis for its actual application in sulfide - containing wastewater treatment. |
| Pages | 22 |
| Language | 中文 |
| Document Type | 学位论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/3909 |
| Collection | 中国科学院广州能源研究所 |
| Recommended Citation GB/T 7714 | 廖俐萍. 绿假单胞菌（Chloropseudomonas sp. G102）及其对硫化物转化的研究[D]. 中国科学院广州能源研究所. 中国科学院广州能源研究所,1994. |
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