Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
One pot cascade biosynthesis of D-allulose from D-glucose and its kinetic modelling | |
Chen, Xiaoyan1; Wang, Fan2; Yu, Qiang3; Liu, Shijie4; Wang, Wen1; Zhang, Yu1![]() ![]() ![]() | |
2022-02-02 | |
发表期刊 | CHEMICAL ENGINEERING SCIENCE
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ISSN | 0009-2509 |
卷号 | 248页码:7 |
通讯作者 | Yu, Qiang(yuqiang@ms.giec.ac.cn) ; Liu, Shijie(sliu@esf.edu) |
摘要 | One pot cascade of enzymatic catalysis was applied for u-allulose synthesis from ID-glucose by the combination of ID-glucose isomerase (GI) from Escherichia coli MG1655 and u-allulose 3-epimerase (DAE) from Agrobacterium tumefaciens C58. The synthesis process was investigated by characterizing the initial substrate concentration, molar ratios of GI and DAE, the enzyme loading, and contents of cobalt and magnesium ions. The increase of enzyme loading and the molar ratios of GI vs DAE caused the raising of u-allulose yield from 4% to 9%, verifying that the generation rate of D-fructose was an essential factor for rare sugar conversion improvement. The optimal enzyme loadings required for reactions with substrate concentrations of 100 g L-1 and 200 g L-1 were 60 U and 200 U. Kinetics stimulation was conducted to reveal the underlying relationship within the three sugars converted by dual-enzyme, with the fitting curve validated the reliability of the model. (C) 2021 Elsevier Ltd. All rights reserved. |
关键词 | D-allullose Dual-enzyme cascade Kinetics stimulation Process optimization |
DOI | 10.1016/j.ces.2021.117167 |
关键词[WOS] | D-PSICOSE 3-EPIMERASE ; D-FRUCTOSE ; ISOMERASE ; TAGATOSE ; MONOSACCHARIDE ; COEXPRESSION ; HYDROGEN ; ENZYME |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[51876206] ; Natural Science Foundation of Guangdong Province[2018A030313012] ; Science and Technology Program of Guangzhou, China[202103000011] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA21050400] ; Young Top-notch Talent of Guangdong Province, China[2016TQ03N647] ; DNL Cooperation Fund, CAS[DNL180305] ; Youth Innovation Promotion Association, CAS[2015289] ; Municipal Science and Technology Project of Guangzhou (China)[201804010081] ; Provincial Science and Technology Project of Guangdong (China)[2017A010105018] ; program of Key Laboratory of Renewable Energy, CAS (China)[E029010801] |
WOS研究方向 | Engineering |
项目资助者 | National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province ; Science and Technology Program of Guangzhou, China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Young Top-notch Talent of Guangdong Province, China ; DNL Cooperation Fund, CAS ; Youth Innovation Promotion Association, CAS ; Municipal Science and Technology Project of Guangzhou (China) ; Provincial Science and Technology Project of Guangdong (China) ; program of Key Laboratory of Renewable Energy, CAS (China) |
WOS类目 | Engineering, Chemical |
WOS记录号 | WOS:000709977400005 |
出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.giec.ac.cn/handle/344007/35140 |
专题 | 中国科学院广州能源研究所 |
通讯作者 | Yu, Qiang; Liu, Shijie |
作者单位 | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.South China Agr Univ, Guangdong Engn Technol Res Ctr Agr & Forestry Bio, Inst Biomass Engn, Key Lab Energy Plants Resource & Utilizat,Minist, Guangzhou 510642, Peoples R China 4.SUNY Syracuse, Dept Chem Engn, Coll Environm Sci & Forestry, Syracuse, NY 13210 USA |
第一作者单位 | 中国科学院广州能源研究所 |
推荐引用方式 GB/T 7714 | Chen, Xiaoyan,Wang, Fan,Yu, Qiang,et al. One pot cascade biosynthesis of D-allulose from D-glucose and its kinetic modelling[J]. CHEMICAL ENGINEERING SCIENCE,2022,248:7. |
APA | Chen, Xiaoyan.,Wang, Fan.,Yu, Qiang.,Liu, Shijie.,Wang, Wen.,...&Yuan, Zhenhong.(2022).One pot cascade biosynthesis of D-allulose from D-glucose and its kinetic modelling.CHEMICAL ENGINEERING SCIENCE,248,7. |
MLA | Chen, Xiaoyan,et al."One pot cascade biosynthesis of D-allulose from D-glucose and its kinetic modelling".CHEMICAL ENGINEERING SCIENCE 248(2022):7. |
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