Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237 | |
Liu, Yunyun1,2; Xie, Xianli1; Liu, Wenhuan1; Xu, Huijuan2; Cao, Yunqi1 | |
2020-11-01 | |
发表期刊 | BIORESOURCES |
ISSN | 1930-2126 |
卷号 | 15期号:4页码:8355-8368 |
通讯作者 | Xu, Huijuan(xuhj@ms.giec.ac.cn) |
摘要 | A consolidated bioprocessing (CBP) using Clostridium thermocellum DSM 1237 for bioethanol production in anaerobic bottles and a 3-L fermenter from biomass was investigated. The effects of key operational parameters including different carbon sources, temperature, and substrates on the metabolic performance of the strain were firstly evaluated. It was found that ethanol yield reached 0.60 g/L with a cell biomass of 0.80 g/g at the optimal temperature of 60 degrees C with 0.5% (w/v) cellobiose. Further experiments indicated that sugarcane bagasse (SCB) could be utilized to efficiently culture this strain. Ethanol yield reached 0.68 g/L (65.8% of theoretical yield) from alkali-pretreated SCB. In the subsequent 3-L fermenter trial, the maximum ethanol 0.86 g/L (83.3% of theoretical yield) was achieved, with enzymes enriched in both cellulase and xylanase. The CBP provided enzymes on-site and integrated hydrolysis and fermentation in one-step, which might be an effective approach for economic bioethanol production. |
关键词 | Clostridium thermocellum Consolidate bioprocess Bioethanol Sugarcane bagasse Fementer |
DOI | 10.15376/biores.15.4.8355-8368 |
关键词[WOS] | CELLULOSIC ETHANOL |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Program of Science & Technology Planning Project of Xi' an city[20193039YF027NS027] ; Natural Science Foundation of Shaanxi Province[2018JQ5134] ; National Natural Science Foundation of China[51876206] ; National Key Research and Development Program[2019YFB1503904] ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources[SKLCUSA-b201802] ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion[Y907k51001] |
WOS研究方向 | Materials Science |
项目资助者 | Program of Science & Technology Planning Project of Xi' an city ; Natural Science Foundation of Shaanxi Province ; National Natural Science Foundation of China ; National Key Research and Development Program ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion |
WOS类目 | Materials Science, Paper & Wood |
WOS记录号 | WOS:000583802900074 |
出版者 | NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.giec.ac.cn/handle/344007/34847 |
专题 | 中国科学院广州能源研究所 |
通讯作者 | Xu, Huijuan |
作者单位 | 1.Shaanxi Univ Sci & Technol, Coll Mech & Elect Engn, Xian 710021, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China |
第一作者单位 | 中国科学院广州能源研究所 |
推荐引用方式 GB/T 7714 | Liu, Yunyun,Xie, Xianli,Liu, Wenhuan,et al. Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237[J]. BIORESOURCES,2020,15(4):8355-8368. |
APA | Liu, Yunyun,Xie, Xianli,Liu, Wenhuan,Xu, Huijuan,&Cao, Yunqi.(2020).Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237.BIORESOURCES,15(4),8355-8368. |
MLA | Liu, Yunyun,et al."Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237".BIORESOURCES 15.4(2020):8355-8368. |
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