A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics

doi:10.1186/s40643-021-00429-8

GIEC OpenIR

	A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics
	Zhang,Jun 1,2,4; Luo,Wen 1; Wang,Zhiyuan 1; Chen,Xiaoyan 1,4; Lv,Pengmei 1; Xu,Jingliang 1,3
	2021-08-19
发表期刊	Bioresources and Bioprocessing
卷号	8 期号:1
通讯作者	Lv,Pengmei(lvpm@ms.giec.ac.cn) ; Xu,Jingliang(xujl@zzu.edu.cn)
摘要	AbstractTo develop an economically feasible fermentation process, this study designed a novel bioprocess based on the co-culture of engineered Bacillus subtilis and Escherichia coli for the co-production of extracellular D-psicose and intracellular lipase. After optimizing the co-culture bioprocess, 11.70?g/L of D-psicose along with 16.03 U/mg of lipase was obtained; the glucose and fructose were completely utilized. Hence, the conversion rate of D-psicose reached 69.54%. Compared with mono-culture, lipase activity increased by 58.24%, and D-psicose production increased by 7.08%. In addition, the co-culture bioprocess was explored through metabolomics analysis, which included 168 carboxylic acids and derivatives, 70 organooxygen compounds, 34 diazines, 32 pyridines and derivatives, 30 benzene and substituted derivatives, and other compounds. It also could be found that the relative abundance of differential metabolites in the co-culture system was significantly higher than that in the mono-culture system. Pathway analysis revealed that, tryptophan metabolism and β-alanine metabolism had the highest correlation and played an important role in the co-culture system; among them, tryptophan metabolism regulates protein synthesis and β-alanine metabolism, which is related to the formation of metabolic by-products. These results confirm that the co-cultivation of B. subtilis and E. coli can provide a novel idea for D-psicose and lipase biorefinery, and are beneficial for the discovery of valuable secondary metabolites such as turanose and morusin.
关键词	D-Psicose Lipase Escherichia coli Bacillus subtilis Co-culture Metabolomics
DOI	10.1186/s40643-021-00429-8
语种	英语
WOS记录号	BMC:10.1186/s40643-021-00429-8
出版者	Springer Singapore
引用统计
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/33652
专题	中国科学院广州能源研究所
通讯作者	Lv,Pengmei; Xu,Jingliang
作者单位	1.Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development 2.Guangdong Ocean University; College of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution 3.Zhengzhou University; School of Chemical Engineering 4.University of Chinese Academy of Sciences
推荐引用方式 GB/T 7714	Zhang,Jun,Luo,Wen,Wang,Zhiyuan,et al. A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics[J]. Bioresources and Bioprocessing,2021,8(1).
APA	Zhang,Jun,Luo,Wen,Wang,Zhiyuan,Chen,Xiaoyan,Lv,Pengmei,&Xu,Jingliang.(2021).A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics.Bioresources and Bioprocessing,8(1).
MLA	Zhang,Jun,et al."A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics".Bioresources and Bioprocessing 8.1(2021).