Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method | |
Zhao, Cheng2,4,6; Yao, Qing-Song2,7; Wang, Can1,2,3,5; Luo, Mu-Tan2,4; Huang, Chao1,2,3,5; Chen, Xue-Fang1,2,3,5; Tian, Lan-Lan2,4; Huang, Qian-Lin2,4; Xiong, Lian1,2,3,5 ; Li, Hai-Long1,2,3,5; Chen, Xin-De1,2,3,5
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| 2021-02-01 | |
| Source Publication | JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN
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| ISSN | 0253-5106 |
| Volume | 43Issue:1Pages:80-87 |
| Corresponding Author | Huang, Chao(huangchao@ms.giec.ac.cn) ; Chen, Xin-De(cxd_cxd@hotmail.com) |
| Abstract | To avoid complex procedures in measurement of lipid content of oleaginous yeast especially for that can accumulate microbial lipid in lignocellulosic hydrolysates, fluorescent method using Nile Red as fluorescent dye was applied to measure lipid content of oleaginous yeast Trichosporon dermatis. The fluorescent method was built by fitting of lipid content identified by both conventional gravimetric method and fluorescence intensity of oleaginous yeast. Within the range of lipid content measured, the fitting curves showed linear relationship with good correlation coefficient (R-2=0.95), showing this method is suitable for measuring lipid content of T. dermatis in the simulated medium. To evaluate the applicability of this method for lipid fermentation using lignocellulosic acid hydrolysates as substrate, T. dermatis was cultured in corncob acid hydrolysate and rice straw acid hydrolysate and then its lipid content measured by both fluorescent method and gravimetric method were compared. The results showed that the lipid content measured by these two methods were close, therefore, this method was promising for the application in lipid fermentation in lignocellulosic acid hydrolysates. |
| Keyword | Microbial lipid Fluorescent method Lipid content Trichosporon dermatis Lignocellulosic hydrolysates |
| WOS Keyword | NILE RED ; INTRACELLULAR LIPIDS ; OIL PRODUCTION ; MICROALGAE |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[21606229] ; project of Pearl River S&T Nova Program of Guangzhou[201610010014] ; Science and Technology Project of Guangdong Province[2016A010105016] ; Foundation Project of Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development[y809jj1001] ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences[y407r41001] |
| WOS Research Area | Chemistry |
| Funding Organization | National Natural Science Foundation of China ; project of Pearl River S&T Nova Program of Guangzhou ; Science and Technology Project of Guangdong Province ; Foundation Project of Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences |
| WOS Subject | Chemistry, Multidisciplinary |
| WOS ID | WOS:000647273100010 |
| Publisher | CHEM SOC PAKISTAN |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/33102 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Huang, Chao; Chen, Xin-De |
| Affiliation | 1.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Guangzhou Inst Energy Convers, R&D Ctr Xuyi Attapulgite Appl Technol, Xuyi 211700, Peoples R China 6.South China Inst Environm Sci, Guangdong Key Lab Water & Air Pollut Control, Minist Ecol & Environm China, Guangzhou 510535, Peoples R China 7.Univ Sci & Technol China, Nano Sci & Technol Inst, Suzhou 215123, Peoples R China |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zhao, Cheng,Yao, Qing-Song,Wang, Can,et al. Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method[J]. JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN,2021,43(1):80-87. |
| APA | Zhao, Cheng.,Yao, Qing-Song.,Wang, Can.,Luo, Mu-Tan.,Huang, Chao.,...&Chen, Xin-De.(2021).Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method.JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN,43(1),80-87. |
| MLA | Zhao, Cheng,et al."Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method".JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN 43.1(2021):80-87. |
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