Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production | |
Miao, Changlin1,2,3; Li, Huiwen1,2,3 ; Zhuang, Xinshu1,2,3; Wang, Zhongming1,2,3; Yang, Lingmei1,2,3; Lv, Pengmei1,2,3,4; Luo, Wen1,2,3
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| 2019-09-22 | |
| Source Publication | RSC ADVANCES
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| Volume | 9Issue:51Pages:29665-29675 |
| Corresponding Author | Yang, Lingmei(yanglm@ms.giec.ac.cn) ; Lv, Pengmei(lvpm@ms.giec.ac.cn) |
| Abstract | In this work, porous cross-linked enzyme aggregates (p-CLEAs) were synthesized by the in situ co-precipitation method using CaCO3 microparticles as templates. The preparation procedure involved the immobilization of crude lipase as CLEAs via precipitation with ammonium sulfate and entrapping these lipase molecules into the CaCO3 templates, followed by DTT (dithiothreitol)-induced assembly of lipase molecules to form lipase microparticles (lipase molecules were assembled into microparticles internally using disulfide bonds within the lipase molecules as the molecular linkers and stimulated by dithiothreitol); finally, the removal of CaCO3 templates was performed by EDTA to form pores in CLEAs. The scanning electron microscopy analysis of p-CLEAs showed a porous structure. p-CLEAs showed obvious improvement in thermal stability (after incubation at 65 degrees C, p-CLEAs lipase retained 86% relative activity, while free lipase retained only 33.67%) and pH stability (p-CLEAs relative activity was over 90% while for free lipase, the relative activity ranged from 72% to 89% from pH 6 to 9) than free lipase and could hold relatively high activity retention without activity loss at 4 degrees C for more than six months. The application of p-CLEAs in producing biodiesel showed a higher degree of conversion. The conversion of fatty acid methyl ester (FAME) was 89.7%; this value was higher by approximately 7.4% compared to that of the conventional CLEAs under the optimized conditions of a methanol-oil molar ratio of 6 : 1, with a p-CLEAs lipase dose of 20% and water content of 3% at 45 degrees C for 24 h. The FAME conversion remained greater than 70% even after reusing the p-CLEAs lipase for 8 reactions. The results demonstrated that the p-CLEAs lipase is suitable for applications in the preparation of biodiesel. |
| DOI | 10.1039/c9ra04365a |
| WOS Keyword | ENZYME AGGREGATES CLEAS ; IMMOBILIZATION ; STABILITY ; OIL ; MICROSPHERES ; PARTICLES ; PAPAIN |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key RD Program[2017YFD0601003] ; National Nature Science Foundation of China[21506217] ; Natural Science Foundation of Guangdong Province[2016A030308004] ; Open Fund of the Key Laboratory of Renewable Energy, Chinese Academy of Sciences[Y707j91001] |
| WOS Research Area | Chemistry |
| Funding Organization | National Key RD Program ; National Nature Science Foundation of China ; Natural Science Foundation of Guangdong Province ; Open Fund of the Key Laboratory of Renewable Energy, Chinese Academy of Sciences |
| WOS Subject | Chemistry, Multidisciplinary |
| WOS ID | WOS:000487364400022 |
| Publisher | ROYAL SOC CHEMISTRY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/25644 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Yang, Lingmei; Lv, Pengmei |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Key Lab New & Renewable Energy Res & De, Guangzhou 510640, Guangdong, Peoples R China 4.Collaborat Innovat Ctr Biomass Energy, Zhengzhou 450002, Henan, Peoples R China |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Miao, Changlin,Li, Huiwen,Zhuang, Xinshu,et al. Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production[J]. RSC ADVANCES,2019,9(51):29665-29675. |
| APA | Miao, Changlin.,Li, Huiwen.,Zhuang, Xinshu.,Wang, Zhongming.,Yang, Lingmei.,...&Luo, Wen.(2019).Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production.RSC ADVANCES,9(51),29665-29675. |
| MLA | Miao, Changlin,et al."Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production".RSC ADVANCES 9.51(2019):29665-29675. |
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