Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Novel Sanger's Reagent-like Styrene Polymer for the Immobilization of Burkholderia cepacia Lipase | |
Fu, Junying1,2; Wang, Zhiyuan1,2; Luo, Wen1,2 ; Xing, Shiyou1,2; Lv, Pengmei1,2,3; Wang, Zhongming1,2; Yuan, Zhenhong1,2,3
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| 2018-09-19 | |
| Source Publication | ACS APPLIED MATERIALS & INTERFACES
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| ISSN | 1944-8244 |
| Volume | 10Issue:37Pages:30973-30982 |
| Corresponding Author | Wang, Zhiyuan(wangzy@ms.giec.ac.cn) ; Lv, Pengmei(lvpm@ms.giec.ac.cn) |
| Abstract | Sanger's reaction, which was originally developed for amino acid detection, was utilized for enzyme immobilization. The newly synthesized polymer support, which was called polymer NO2-4-fluorostyrene-divinylbenzene (pNFD), was embedded with a Sanger's reagent-like functional group for immobilizing enzymes covalently under mild reaction conditions. Using Burkholderia cepacia lipase (BCL) as the target enzyme, the immobilization efficiency and activity of pNFD BCL reached as high as 1.2 mg.g(-1) and 33.21 U.g(-1) (a specific activity of 27 67S U.g(-1)), respectively, realizing 90% activity recovery. It also improved the optimal reaction temperature of BCL from 40 to 65 degrees C, under which its full activity could be retained for 4 h. The new carrier also widened the pH-adaptive range of BCL as 6.5-10.0, allowing the lipase to operate normally in weak acid environment. Reusability of pNFD BCL was significantly improved as almost no activity and/or enantioselectivity loss was observed in 200 h of triglyceride hydrolysis reaction and 17 batches of (R,S)-1phenylethanol resolution reaction. |
| Keyword | Sanger's reagent immobilization lipase hydrolysis enantioselectivity |
| DOI | 10.1021/acsami.8609225 |
| WOS Keyword | ENZYME IMMOBILIZATION ; ORGANIC-SOLVENTS ; POTENTIAL APPLICATIONS ; HYDROPHOBIC SUPPORTS ; CATALYTIC-PROPERTIES ; BIODIESEL PRODUCTION ; MESOPOROUS SILICA ; RESOLUTION ; STABILITY ; MEDIA |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[21576260] ; Special Funds of Applied Science and Technology Research of Guangdong Province[2015B020241002] ; Science and Technology Planning Project of Guangdong Province[2016A010104008] ; Natural Science Foundation of Guangdong Province[2015A030313720] ; Natural Science Foundation of Guangdong Province[2017A010104010] |
| WOS Research Area | Science & Technology - Other Topics ; Materials Science |
| Funding Organization | National Natural Science Foundation of China ; Special Funds of Applied Science and Technology Research of Guangdong Province ; Science and Technology Planning Project of Guangdong Province ; Natural Science Foundation of Guangdong Province |
| WOS Subject | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| WOS ID | WOS:000445439900008 |
| Publisher | AMER CHEMICAL SOC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/23886 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Wang, Zhiyuan; Lv, Pengmei |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangdong Key Lab New & Renewable Energy Res & De, Guangzhou 510640, Guangdong, Peoples R China 3.Collaborat Innovat Ctr Biomass Energy, Zhengzhou 450002, Henan, Peoples R China |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Fu, Junying,Wang, Zhiyuan,Luo, Wen,et al. Novel Sanger's Reagent-like Styrene Polymer for the Immobilization of Burkholderia cepacia Lipase[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(37):30973-30982. |
| APA | Fu, Junying.,Wang, Zhiyuan.,Luo, Wen.,Xing, Shiyou.,Lv, Pengmei.,...&Yuan, Zhenhong.(2018).Novel Sanger's Reagent-like Styrene Polymer for the Immobilization of Burkholderia cepacia Lipase.ACS APPLIED MATERIALS & INTERFACES,10(37),30973-30982. |
| MLA | Fu, Junying,et al."Novel Sanger's Reagent-like Styrene Polymer for the Immobilization of Burkholderia cepacia Lipase".ACS APPLIED MATERIALS & INTERFACES 10.37(2018):30973-30982. |
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