Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis | |
| Li, Hailong1,3,4; Wu, Hongli2; Jiang, Fengjiao2; Wu, Jinlian2; Xue, Yong2; Gan, Lihui2; Liu, Jian2; Long, Minnan2 | |
| 2018 | |
| Source Publication | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| ISSN | 0273-2289 |
| Volume | 184Issue:1Pages:228-238 |
| Corresponding Author | Liu, Jian(jianliu@xmu.edu.cn) ; Long, Minnan(longmn@xmu.edu.cn) |
| Abstract | A gene encoding glycoside hydrolase family 11 xylanase (HoXyn11B) from Hypocrea orientalis EU7-22 was expressed in Pichia pastoris with a high activity (413 IU/ml). HoXyn11B was partly N-glycosylated and appeared two protein bands (19-29 kDa) on SDS-PAGE. The recombinant enzyme exhibited optimal activity at pH 4.5 and 55 A degrees C, and retained more than 90% of the original activity after incubation at 50 A degrees C for 60 min. The determined apparent K (m) and V (max) values using beechwood xylan were 10.43 mg/ml and 3246.75 IU/mg, respectively. The modes of action of recombinant HoXyn11B on xylo-oligosaccharides (XOSs) and beechwood xylan were investigated by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), which indicated that the modes of action of HoXyn11B are different from HoXyn11A since it is able to release a significant amount of xylose from various substrates. This study provides an opportunity to better understand the hydrolysis mechanisms of xylan by xylanases from Trichoderma. |
| Keyword | Heterologous expression Pichia pastoris Xylan hydrolysis Xylanases Hypocrea orientalis Xylo-oligosaccharides |
| DOI | 10.1007/s12010-017-2532-2 |
| WOS Keyword | PICHIA-PASTORIS ; ENZYMATIC-HYDROLYSIS ; RECOMBINANT XYLANASE ; XYLOOLIGOSACCHARIDES ; OLIGOSACCHARIDES ; MONOSACCHARIDES ; HEMICELLULOSES ; PURIFICATION ; PROTEINS ; BINDING |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Natural Science Foundation of Guangdong Province, China[2016A030310124] ; National Natural Science Foundation of China[31600475] ; project of Guangzhou Science and Technology[201707010241] ; research fund from the Xiamen Southern Oceanographic Center[14GZP59HJ29] ; Fujian Provincial Department of Ocean and Fisheries[2015-27] ; President Fund of Xiamen University[20720150090] |
| WOS Research Area | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| Funding Organization | Natural Science Foundation of Guangdong Province, China ; National Natural Science Foundation of China ; project of Guangzhou Science and Technology ; research fund from the Xiamen Southern Oceanographic Center ; Fujian Provincial Department of Ocean and Fisheries ; President Fund of Xiamen University |
| WOS Subject | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS ID | WOS:000419607700018 |
| Publisher | HUMANA PRESS INC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/22884 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Liu, Jian; Long, Minnan |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.Xiamen Univ, Coll Energy, Xiamen 361102, Peoples R China 3.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 4.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Li, Hailong,Wu, Hongli,Jiang, Fengjiao,et al. Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2018,184(1):228-238. |
| APA | Li, Hailong.,Wu, Hongli.,Jiang, Fengjiao.,Wu, Jinlian.,Xue, Yong.,...&Long, Minnan.(2018).Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,184(1),228-238. |
| MLA | Li, Hailong,et al."Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 184.1(2018):228-238. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment