Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry | |
| Huang, Chao1,3,4; Zhu, Ding-he2; Wu, Hong1,2; Lou, Wen-yong1; Zong, Min-hua1 | |
| 2014-03-01 | |
| Source Publication | PROCESS BIOCHEMISTRY
 |
| Volume | 49Issue:3Pages:395-401 |
| Abstract | The effect of ten typical organic acids and five aldehydes present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans was evaluated by flow cytometry. Overall, organic acids affected the cell membrane integrity of T. fermentans more significantly than that of aldehydes albeit aldehydes are more toxic to T. fermentans. The PI (Propidium Iodide) uptake rate of T. fermentans' cells gradually decreased as fermentation going on, indicating that T. fermentans could overcome the inhibition of organic acids or aldehydes by adaption. Interestingly, in some cases, the effect of organic acids or aldehydes on the cell membrane integrity of T. fermentans was well related to their hydrophobicity. However, for the outliers, no obvious similar phenomena were observed. Thus, the attack on hydrophobic sites of cell membrane was not the only determinant for the damage of organic acids or aldehydes on cell membrane integrity of T. fermentans. (C) 2013 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Trichosporon Fermentans Organic Acid Aldehyde Cell Membrane Integrity Flow Cytometry Lignocellulosic Hydrolysate |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1016/j.procbio.2013.12.007 |
| WOS Subject Extended | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| WOS Keyword | ETHANOLOGENIC ESCHERICHIA-COLI ; SACCHAROMYCES-CEREVISIAE ; LIPID-ACCUMULATION ; OIL PRODUCTION ; ORGANIC-ACIDS ; GROWTH ; CONVERSION ; ALDEHYDES |
| Indexed By | SCI |
| Language | 英语 |
| WOS Subject | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| WOS ID | WOS:000334482300006 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/10708 |
| Collection | 中国科学院广州能源研究所 |
| Affiliation | 1.S China Univ Technol, State Key Lab Pulp & Paper Engn, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China 2.S China Univ Technol, Prov Key Lab Green Proc Technol & Prod Safety Nat, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China 3.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Huang, Chao,Zhu, Ding-he,Wu, Hong,et al. Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry[J]. PROCESS BIOCHEMISTRY,2014,49(3):395-401. |
| APA | Huang, Chao,Zhu, Ding-he,Wu, Hong,Lou, Wen-yong,&Zong, Min-hua.(2014).Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry.PROCESS BIOCHEMISTRY,49(3),395-401. |
| MLA | Huang, Chao,et al."Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry".PROCESS BIOCHEMISTRY 49.3(2014):395-401. |
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