Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Characterization of direct cellulase immobilization with superparamagnetic nanoparticles | |
| Xu, Jingliang1; Huo, Shuhao2; Yuan, Zhenhong1; Zhang, Yu1; Xu, Huijuan1; Guo, Ying1; Liang, Cuiyi1; Zhuang, Xinshu1 | |
| 2011-03-01 | |
| Source Publication | BIOCATALYSIS AND BIOTRANSFORMATION
 |
| Volume | 29Issue:2-3Pages:71-76 |
| Abstract | Methods of cellulase immobilization on magnetic particles via glutaraldehyde binding were studied. The binding was confirmed by transmission electronic microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and vibrating sample magnetometry (VSM). Samples analyzed by TEM and XRD showed that the magnetic particles with or without bound cellulase were all nanosized particles with a mean diameter of 11.5 nm, and the binding process did not cause significant changes in particle size and structure. Analysis by FTIR showed that the binding of cellulase to the magnetic nanoparticles might be via covalent bonding between residual amine groups on Fe(3)O(4) nanoparticles and amine groups of the cellulase. The VSM analysis showed that magnetic nanoparticles with or without bound cellulase were all superparamagnetic. The immobilized cellulase had a wider pH and temperature range and improved storage stability compared with the free enzyme. Determination of the Michaelis constants revealed that the immobilized cellulase had a greater affinity for the cellulosic substrate than the free enzyme. The immobilized cellulase showed better performance on hydrolysis of steam-exploded corn stalks than of bleached sulfite bagasse pulp. |
| Subtype | Article |
| Keyword | Cellulase Direct Immobilization Superparamagnetic Nanoparticles Glutaraldehyde |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.3109/10242422.2011.566326 |
| WOS Subject Extended | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS Keyword | DIRECT BINDING PROCEDURE ; MAGNETIC NANOPARTICLES ; PARTICLES ; DEHYDROGENASE ; STABILITY |
| Indexed By | SCI |
| Language | 英语 |
| WOS Subject | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS ID | WOS:000290868200004 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/10413 |
| Collection | 中国科学院广州能源研究所 |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Guangdong, Peoples R China 2.Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Xu, Jingliang,Huo, Shuhao,Yuan, Zhenhong,et al. Characterization of direct cellulase immobilization with superparamagnetic nanoparticles[J]. BIOCATALYSIS AND BIOTRANSFORMATION,2011,29(2-3):71-76. |
| APA | Xu, Jingliang.,Huo, Shuhao.,Yuan, Zhenhong.,Zhang, Yu.,Xu, Huijuan.,...&Zhuang, Xinshu.(2011).Characterization of direct cellulase immobilization with superparamagnetic nanoparticles.BIOCATALYSIS AND BIOTRANSFORMATION,29(2-3),71-76. |
| MLA | Xu, Jingliang,et al."Characterization of direct cellulase immobilization with superparamagnetic nanoparticles".BIOCATALYSIS AND BIOTRANSFORMATION 29.2-3(2011):71-76. |
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